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Highly Sensitive Analysis of Organic Acids by HPLC-UV [AN0066]

Organic acids such as malic, ascorbic and citric acid, are commonly found in food and beverage products. Derived by both natural biochemical
processes and added as preservatives/stabilisers, organic acids contribute to the sensory properties of food and drink, including both aroma and taste. The monitoring of these organic acids is essential for both quality control during production of said products but also for evaluating food authenticity and purity. Although regulations vary widely, regulations are in place to prevent the bulk use of these ingredients.
SCION Instruments developed a HPLC method for the simultaneous identification of ten organic acids, using a single wavelength by UV detection. UV detection is possible by the detection of absorption via the carboxyl groups of the organic acids. Additionally, the use of a low carbon octadecylsilyl (ODS) column reduced the hydrophobicity of the silica surface, providing a stable analysis for the separation of high polarity compounds, such as organic acids, in a 100% aqueous solution.

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HPLC Analysis of Fat-Soluble Vitamins [AN0061]

Vitamins are critical compounds which are essential for normal metabolism function. They are naturally found in many foods but are also often added to processed food products. Additionally, vitamin supplements are a growing trend among people whose diet is restricted. Vitamins are categorised into two groups; water soluble and fat soluble. The most common fat-soluble vitamin supplements are Vitamin A (retinol), Vitamin A Acetate (Retinol Acetate), Vitamin D2 (Ergocalciferol), Vitamin D3 (Cholecalciferol), Vitamin E (dl-α-tocopherol), Vitamin E Acetate (dlα- tocopherol acetate) and Vitamin K1 (Phylloquinone).
SCION Instruments developed a qualitative method for the simultaneous identification of seven fat-soluble vitamins. As individual vitamins are unstable, it is recommended for quantitative analysis that each vitamin components is prepared and analysed with individual analytical methods.

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HPLC Analysis of Water-Soluble Vitamins [AN0060]

Vitamins are critical compounds which are essential for normal metabolism function. They are naturally found in many foods but are also often added to processed food products. Additionally, vitamin supplements are a growing trend among people whose diet is restricted. Vitamins are separated into two groups; water soluble and fat soluble. The most common water-soluble vitamin supplements are Thiamine (B1), Pyridoxine (B6), Cyanocobalamin (B12), Riboflavin (B2), Niacin (B3) Nicotinamide, Ascorbic Glucoside, Vitamin C and Erythorbic Acid.
Routine analysis of water-soluble vitamins can be challenging due to the unstable nature of the target analytes. Many factors can affect vitamin stability such as exposure to heat, light, air as well as interactions with other food components. By using reverse phase high pressure liquid chromatography (HPLC) with Diode Array Detector (DAD) a qualitative method for the detection of water-soluble vitamins was easily developed. For quantitative analysis, separate HPLC methods are recommended due to Vitamin C and Erythorbic Acid instability in which decomposition regularly occurs during sample preparation.

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LC6000 Analysis of Glycosides in Medicines [AN0067]

Glycosides are molecules in which a sugar is bound to another functional group via a glycosidic bond. Glycosides are mainly O-glycosides, sugar derivatives with various physiological activities that are widely distributed in plants. Additionally, glycosides are widely used as components of unrefined/ herbal medications, such as ginseng or Senna.
SCION Instruments developed a qualitative and quantitative method for the analysis of glycosides in medicines by HPLC-Diode Array Detection (DAD). Confirmation is further certified through the comparison of the absorbance spectrum of both analytical standard and sample analysed.

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LC6000_Simultaneous Analysis of Parabens and Phenoxyethanol_2020.1 [AN068]

INTRODUCTION: Parabens are a class of widely used preservatives in cosmetic and pharmaceutical products, primarily due to their bactericidal and fungicidal properties. Parabens are a series of parahydroxybenzoates which are currently under scrutiny due to the possibility that long-term use of products containing parabens can cause hormone disruption and fertility issues, although this has not been confirmed. Methyl phydroxybenzoate, ethyl p-hydroxybenzoate, propyl p-hydroxybenzoate and butyl p-hydroxybenzoate are the most common used parabens in the cosmetic and pharmaceutical industry. Phenoxyethanol is an additional component used as a germicide and antiseptic ingredient in cosmetics. Naturally occurring in plant material such as green tea, phenoxyethanol is often used in tandem with parabens to reduce the number of parabens used during the manufacturing of such products.Monitoring paraben and phenoxyethanol concentrations are a vital part of any cosmetic of pharmaceutical manufacturer.

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Methanol in Crude Oil Analysis According to ASTM D7059-04 [AN0069]

INTRODUCTION: The analysis of methanol in crude oil according to ASTM D 7059: “Standard Test Method for Determination of Methanol in Crude Oils by Multidimensional Gas Chromatography.”
In the production of crude oil, methanol is often added to prevent formation of hydrates (i.e., solid water-hydrocarbon structures) that block transportation via pipelines. However, methanol, as with most oxygenated components, will poison catalysts when the crude oil is converted into other products. Catalysts are very expensive, and high methanol levels add a big cost factor. Therefore, the content of methanol in a crude oil is very important and must be measured accurately at low (ppm) concentrations. Depending on the methanol level, the crude oil can be applied straight or needs extra treatment(s) to reduce the methanol content. The determination of methanol is done via an internal standard method using 1propanol as the internal standard. The measuring range of methanol is specified from 15 to 900 ppm (m/m).

Showing 51 - 60 of 86 results