How To Guide for GC Method Development
GC Method Development
Method development ensures that analytical assays are accurate, precise, and reliable. The process begins with a thorough understanding of the sample, including its composition and properties. Key factors to consider include the sample’s matrix, concentration, quantity, and chemical or physical characteristics. These elements must be evaluated before defining the method parameters.
Once the sample is understood, the next step is to identify the goals of the analysis. What is the purpose of analysing the sample? For instance, are we aiming to quantify the compound, characterize it, or simply detect its presence?
After establishing the goals, we then determine the analysis requirements. This involves selecting the appropriate mode of chromatography, choosing the right detector, deciding on data quantification methods (such as using an internal standard), and defining the desired levels of accuracy and precision.
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James covers the following key topics in his presentation:
• Initial considerations – column choice, liner choice, instrument parameters
• Optimising method – achieving resolution, sensitivity using analytical standard
• Introducing sample matrix – sample prep considerations
• SCION consumables kits overview – perfect for method development
• Validation considerations to ensure good working of method