This application will study the quantification and qualification of FAME within EVOO’s, by GC with a Flame Ionization Detector (FID)

This application can be performed on either the SCION Instruments 8300 GC & 8500 GC platform with 8700 Single Quad Mass Spectrometer (SQMS) and the SCION 8400PRO Autosampler, shown in figure 1. A SCION-FAME column is used for obtaining the best separation of the cis and trans-isomers of most FAME’s.

Biodiesel is produced by transesterification of the parent oil or fat with an alcohol, usually methanol, in the presence of a catalyst, usually potassium hydroxide or sodium hydroxide, or, increasingly, alkoxides. The resulting product can contain not only the desired alkyl ester product but also unreacted starting material mono-, di- and triacylglycerides, residual alcohol and catalyst...

For biodiesel to be used as a motor fuel or blended with petroleum diesel, it must conform to standard specifications (ASTM D 6751 or EN-14214). There are standard GC methods in use today to determine if biodiesel conforms to the standard specifications, one of which is EN-14103, used to determine the ester and linoleic acid methyl ester content...

There has been a growing demand for the analysis of oils, fats and fat containing food products especially surrounding the edible oils market. The most common analysis of such products are the determination of fatty acid methyl esters (including cis and trans isomers and omegas...

In the constant quest for cost reduction, speed of analysis and sample throughput this application outlines the possibilities of doubling sample throughput with existing equipment. A SCION 456 GC was equipped with two identical channels containing a split/splitless injector, a FAME column and a Flame Ionisation Detector (FID). A single SCION 8400 autosampler was used to inject two samples into the two injection ports. The advantages of this approach are two-fold. First and foremost, a doubling of sample throughput is obtained. Secondly, this can be done without adding hardware to the autosampler system; for the second simultaneous injection. This application note focuses on chromatographic performance and system integrity by showing that both channels perform equally well on peak repeatability and peak resolution.

Biodiesel is produced by transesterification of the parent oil or fat with an alcohol, usually methanol, in the presence of a catalyst, usually potassium hydroxide or sodium hydroxide. Alkoxides are now becoming more increasingly popular. The resulting biodiesel product contains not only the desired alkyl ester product but also non or partly reacted starting material mono-, di-, and triacylglycerides, residual alcohol and catalysts. Glycerol is formed as a by-product and separated from the biodiesel in the production process. However, traces of glycerol can be found in the final biodiesel product.