Guidelines for HPLC Column Handling

For best column performance, precautions must be taken when handling a HPLC column.

Before installing a HPLC column, replace the mobile phase within the full HPC system with new mobile phase (always check the miscibility of a buffer with solvent)
Using the flow direction arrow on the column, connect the column inlet to the end of the column
Gradually increase the flow rate of the mobile phase throughout the column
When heating the column e sure to pump the mobile phase at a low flow rate until the specific temperature is reached
Once column flow is established and the column is properly flushed, connect the column outlet to the detector inlet

Flush the column with 3 to 5 times volume of 100% organic solvent (50:50% organic/water for HILIC columns)
If the column oven is heated, turn off the heater and reduce the flow rate to 1/3^rd of the regular flow
When the column oven is at room temperature, turn off pump flow
Remove the inlet and outlet from the column and fit the shipping caps
Gently tighten the caps

Securely store the column in its original packaging
Keep the column in a cool, dark space away from sunlight and extreme temperatures

Download our HPLC Column Guide

For more information and useful guidance download our HPLC Column Guide.