THC Potency Testing Cannabis Using HPLC

Test for THC Potency with SCION

When it comes to THC potency testing, High-Performance Liquid Chromatography (HPLC) is the method of choice. What sets HPLC  apart is its ability to deliver accurate analysis without the need for heating procedures. One of the benefits of using HPLC over GC to test for THC potency is the ability to discriminate between the acidic and non acidic forms of certain cannabinoids (CBD, CBG and 9-THC).

When using GC, the heat of the injector causes the acidic forms to degrade into their nonacidic counterparts, this can be avoided by derivatizing the compounds, but this requires lengthy additional sample preparation. Whilst many regulatory bodies allow for the reporting of the acidic and non acidic forms as a summation this is not always the case.

Cannabis Potency Test. View our app not looking at Cannabis Potency Testing.

Using HPLC for a THC potency test avoids this issue and allows for quantification of each component separately without the requirement of further sample preparation.

This not only saves time but also ensures the integrity of the cannabis sample. In fact, HPLC has also gained a reputation for providing superior accuracy compared to alternative methods. If precision and reliability are what you seek in your potency testing, HPLC is the go-to technique that won’t disappoint.

Potency Test Using SCION Instruments

THC Potency Test. Using HPLC for Potency Testing.

The Scion 6000 Series HPLC aims for confidence in results through outstanding lifetime performance. A robust design maximises uptime and productivity levels whilst minimises cost of operation. The SCION 6000 Series offers an array of automation options for workflow optimisation, making our lab experience the best and easiest possible.

To find out more about using the LC 6000 for potency testing, please get in touch, and a member of our team will be delighted to help.

Explore the full potential of cannabis analysis with SCION.

Related Application Notes

  • Download our app note to learn how to test cannabis potency using HPLC-UV – with testing set to become stricter with governing bodies such as the FDA assessing their guidelines increasing the importance of accurate quantification of these components
  • Find out the method conditions required, how to validate your method to be sure it’s working well and how to prepare different cannabis samples for analysis (CBD oil and marijuana)
  • See the advantage of this technique being able to analyse acidic cannabinoids without the lengthy sample preparation seen in GC methods

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