{"id":57031,"date":"2024-07-09T14:49:28","date_gmt":"2024-07-09T13:49:28","guid":{"rendered":"https:\/\/scioninstruments.com\/analysis-of-fatty-acids-in-fish-oil-supplements-using-scion-gc-fid\/"},"modified":"2025-04-25T09:12:50","modified_gmt":"2025-04-25T08:12:50","slug":"analysis-of-fatty-acids-in-fish-oil-supplements-using-scion-gc-fid","status":"publish","type":"page","link":"https:\/\/scioninstruments.com\/us\/analysis-of-fatty-acids-in-fish-oil-supplements-using-scion-gc-fid\/","title":{"rendered":"Analysis of Fatty Acids in Fish Oil Supplements using SCION GC-FID"},"content":{"rendered":"

Introduction<\/h2>\n

The purpose of this study is to determine the fatty acid content in fish oil using a SCION Instruments 8500 GC with PTV injector, FID and SCION WAX column.<\/p>\n

The analysis of fatty acids in fish oil is a critical application for the food and health industries. Accurate determination of fatty acids is vital for quality control and authenticity of products containing fatty acids\/ omegas. There has been a growing demand for the analysis of oils, fats and fat containing food products especially surrounding the edible oils market.<\/p>\n

Depending on your application a variety of columns can be suitable for FAME analysis as shown in this application note using SCION WAX 25 m x 0.25 mm x 0.2 \u00b5m column. The SCION FAME 100 m x 0.25 mm x 0.2 \u00b5m column is specifically designed for the analysis of complex FAME patterns including the separation and identification of cis\/trans isomers, specifically those of C18:1.
\nThe SCION 8500 GC was used to accurately separate, identify and quantify fatty acids from C4 to C24:1. With enhanced resolution, target fatty acids were detectable down to 0.05%.<\/p>\n

In 2020, The Global Organisation for EPA and DHA Omega-3 Fatty Acids (GOED) specified the SCION Instruments 436-GC with PTV and FID detector as the recommended equipment for getting optimal results for analysis of fatty acids.1 The 8300 GC is the next generation 436 instrument, delivering the same excellent performance. Near 100% recovery was achieved using the SCION 436-GC, which eliminates inter-laboratory variability.<\/p>\n

Experimental<\/h2>\n

Experimental conditions for the fatty acid analysis can be found in Table 1.<\/p>\n

Table 1 Analytical method parameters<\/h6>\n

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Results<\/h2>\n

A calibration standard containing fatty acids C4 to C24:1 was analysed to determine the retention time of each fatty acid. A pharmaceutical grade fish oil supplement was analysed with qualitative and quantitative data analysis performed. A total of 43 fatty acids were detected in the sample.<\/p>\n

Table 2 shows a summary report of omega types detected as the associate mole percentages.<\/p>\n

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Upon the recommendation of the GOED, system repeatability and reproducibility of fatty acids in fish oils was tested using eight different GC instruments in four different laboratories. A total of 56 injections were made with six different laboratory operators. A GLC-68D standard mixture at 100\u00b5g\/mL was used for each test. The results are shown in Table 3 and 4.<\/p>\n

The tests were performed on a SCION-WAX 25m x 0.25mm x 0.20\u00b5m column. The recovery requirements for the fatty acids was 95-105%.<\/p>\n

Conclusions<\/h2>\n

The Global Organisation for EPA and DHA Omega-3 Fatty Acids (GOED) specified the SCION Instruments 436-GC with PTV and FID detector as the recommended equipment for getting optimal results for analysis of fatty acids. The SCION Instrument 8300 and 8500 GC\u2019s are the new generation instruments which show the same great performance for fatty acid analysis in edible oils, including fish oil supplements. Enhanced resolution for complicated samples can be achieved using the SCION WAX column. Excellent repeatability, reproducibility and recovery were observed through proficiency testing using in four different laboratories on eight different systems.<\/p>\n

Acknowledgements<\/h2>\n

Terje Aasoldsen, SAMSI Analytical Application Services, based in Norway, who provided the data and all of their expertise in this application field.
\nMIDI.Inc who provided the qualitative and quantitative data.
\nReferences
\n1. Goedomega3, https:\/\/goedomega3.com\/storage\/app\/media\/technical%20reports\/TGD%202020%2006%2029.pdf, (date accessed 23 April 25).<\/p>\n

Ordering Information<\/h2>\n

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For ordering info on the SCION 8300 and 8500 GC, which offers greater functionality with the option of up to 4 detectors (including MS), please contact your local SCION sales representative.<\/p>\n

For more information, please contact:
\nE: sales-eu@scioninstruments.com
\nW: www.scioninstruments.com<\/p>\n

Download application note- AN176v3 Analysis of Fatty Acids in Fish Oil Supplements using SCION GC-FID<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

Introduction The purpose of this study is to determine the fatty acid content in fish oil using a SCION Instruments 8500 GC with PTV injector, FID and SCION WAX column. The analysis of fatty acids in fish oil is a critical application for the food and health industries. Accurate determination of fatty acids is vital […]<\/p>\n","protected":false},"author":932,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-57031","page","type-page","status-publish","hentry"],"acf":{"rf_hero_height":"default","rf_hero_type":"default","rf_hero_vertpos":"50","rf_hero_horizpos":"50","rf_hero_title":"","rf_hero_title_hide":false,"rf_hero_textcol":"light","rf_hero_bgcol":"white","rf_hero_tint":false,"rf_further_reading_posts":false},"yoast_head":"\nAnalysis of Fatty Acids in Fish Oil Supplements<\/title>\n<meta name=\"description\" content=\"Explore the analysis of fatty acids in fish oil supplements, focusing on methods, benefits, and quality assessment.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" 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