{"id":46571,"date":"2022-04-26T15:59:46","date_gmt":"2022-04-26T14:59:46","guid":{"rendered":"https:\/\/scioninstruments.com\/?page_id=46571"},"modified":"2024-02-02T09:43:37","modified_gmt":"2024-02-02T09:43:37","slug":"multiplexed-pesticide-analysis-by-gc-tqms","status":"publish","type":"page","link":"https:\/\/scioninstruments.com\/us\/multiplexed-pesticide-analysis-by-gc-tqms\/","title":{"rendered":"Multiplexed pesticide analysis by GC-TQMS"},"content":{"rendered":"<h2>Introduction<\/h2>\n<p>As plant protection products, pesticides may be used in food production (growth), storage, and transport. The potential physico-chemical transfer of regulated pesticides\u00a0 varies with the type of pesticide used and the type of food being produced.\u00a0 The maximum residue levels (MRLs) of pesticides established by the European Commission in effort to protect consumer safety differ according to their risk with the majority of MRLs falling between 10-500 \u03bcg\/kg (ppb). For pesticides without an established MRL, the minimum value of 10 ppb is taken.<\/p>\n<p><a href=\"https:\/\/scioninstruments.com\/wp-content\/uploads\/2022\/04\/Figure-0.png\"><img loading=\"lazy\" decoding=\"async\" loading=\"lazy\" class=\"size-full wp-image-46572 alignnone\" src=\"https:\/\/scioninstruments.com\/wp-content\/uploads\/2022\/04\/Figure-0.png\" alt=\"\" width=\"836\" height=\"840\" srcset=\"https:\/\/scioninstruments.com\/wp-content\/uploads\/2022\/04\/Figure-0.png 836w, https:\/\/scioninstruments.com\/wp-content\/uploads\/2022\/04\/Figure-0-300x300.png 300w, https:\/\/scioninstruments.com\/wp-content\/uploads\/2022\/04\/Figure-0-150x150.png 150w, https:\/\/scioninstruments.com\/wp-content\/uploads\/2022\/04\/Figure-0-768x772.png 768w\" sizes=\"auto, (max-width: 836px) 100vw, 836px\" \/><\/a><\/p>\n<p><strong>Figure 1. SCION Instruments\u00a0 8300 GC coupled to a 8900 TQMS with a 8400 Autosampler<\/strong><\/p>\n<p>Traditional GC-TQMS\u00a0analysis of multi-residue pesticides suffers from a lack of sensitivity when many compounds are analysed in a single run , with scan \u00a0\u00a0times below 10ms. In general, longer \u00a0\u00a0\u00a0chromatographic separations are required in order to save\u00a0scan times over 10ms resulting in longer \u00a0analysis time and lower sensitivity.<\/p>\n<p>The high accuracy and speed of analyses using the 8900 GC-TQMS system, is extremely well suited to meet the laboratory necessities of method throughput and sensitivity for rapid determination of target pesticides.<\/p>\n<p>In this application note, we will explore the ultimate performance of the SCION 8900 GC-TQMS for the simultaneous analysis of 450 pesticides without loss of sensitivity or reproducibility.<\/p>\n<h2>Instrumentation<\/h2>\n<p>GC-TQMS of pesticides is a routine application but multiplexing the analysis of multiple pesticides without losing sensitivity can lead to long assay times. The high MRM speed of the 8900 TQMS coupled to the performance of the 8500 GC can overcome this. The method described uses a short (16 m) column oven program with an optimised backflush for both matrix removal and solvent vent (Figure 2).<\/p>\n<p><a href=\"https:\/\/scioninstruments.com\/wp-content\/uploads\/2022\/04\/Figure-1.png\"><img loading=\"lazy\" decoding=\"async\" loading=\"lazy\" class=\"size-full wp-image-46578 alignnone\" src=\"https:\/\/scioninstruments.com\/wp-content\/uploads\/2022\/04\/Figure-1.png\" alt=\"\" width=\"784\" height=\"664\" srcset=\"https:\/\/scioninstruments.com\/wp-content\/uploads\/2022\/04\/Figure-1.png 784w, https:\/\/scioninstruments.com\/wp-content\/uploads\/2022\/04\/Figure-1-300x254.png 300w, https:\/\/scioninstruments.com\/wp-content\/uploads\/2022\/04\/Figure-1-768x650.png 768w\" sizes=\"auto, (max-width: 784px) 100vw, 784px\" \/><\/a> <a href=\"https:\/\/scioninstruments.com\/wp-content\/uploads\/2022\/04\/Figure-1b.png\"><img loading=\"lazy\" decoding=\"async\" loading=\"lazy\" class=\"alignnone wp-image-46584 size-large\" src=\"https:\/\/scioninstruments.com\/wp-content\/uploads\/2022\/04\/Figure-1b-1024x254.png\" alt=\"\" width=\"1024\" height=\"254\" srcset=\"https:\/\/scioninstruments.com\/wp-content\/uploads\/2022\/04\/Figure-1b-1024x254.png 1024w, https:\/\/scioninstruments.com\/wp-content\/uploads\/2022\/04\/Figure-1b-300x74.png 300w, https:\/\/scioninstruments.com\/wp-content\/uploads\/2022\/04\/Figure-1b-768x191.png 768w, https:\/\/scioninstruments.com\/wp-content\/uploads\/2022\/04\/Figure-1b.png 1052w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/a><\/p>\n<p><strong>Figure 2.\u00a0Simple GC\u00a0 program for rapid pesticide analysis<\/strong><\/p>\n<p>In a standard method, 20 compounds can be analysed, using less than 50 transitions and a compound scan time of 111ms (Figure 3A). With the 8900 TQMS, 450 compounds with more than 1100 mass transitions can be analysed with a compound scan time of 4ms (Figure 3B).<\/p>\n<p><a href=\"https:\/\/scioninstruments.com\/wp-content\/uploads\/2022\/04\/Figure-2.jpg\"><img loading=\"lazy\" decoding=\"async\" loading=\"lazy\" class=\"size-full wp-image-46590 alignnone\" src=\"https:\/\/scioninstruments.com\/wp-content\/uploads\/2022\/04\/Figure-2.jpg\" alt=\"\" width=\"775\" height=\"640\" srcset=\"https:\/\/scioninstruments.com\/wp-content\/uploads\/2022\/04\/Figure-2.jpg 775w, https:\/\/scioninstruments.com\/wp-content\/uploads\/2022\/04\/Figure-2-300x248.jpg 300w, https:\/\/scioninstruments.com\/wp-content\/uploads\/2022\/04\/Figure-2-768x634.jpg 768w\" sizes=\"auto, (max-width: 775px) 100vw, 775px\" \/><\/a><\/p>\n<p><b>Figure 3.\u00a0 Increasing MRM does not impede sensitivity<\/b><\/p>\n<p>Despite the increase in number of components measured, (Figure 3), the SCION 8900 TQMS maintains good accuracy and linearity at scan times of 4ms. The ion\u00a0 ratio stability is maintained when switched from 40ms to 7ms scan time. This is maintained even at the lowest concentration of calibration standards (down to 1ppb). Reproducibility is maintained over multiple runs.<\/p>\n<p><a href=\"https:\/\/scioninstruments.com\/wp-content\/uploads\/2022\/04\/Figure-3.jpg\"><img loading=\"lazy\" decoding=\"async\" loading=\"lazy\" class=\"size-large wp-image-46596 alignnone\" src=\"https:\/\/scioninstruments.com\/wp-content\/uploads\/2022\/04\/Figure-3-1024x352.jpg\" alt=\"\" width=\"1024\" height=\"352\" srcset=\"https:\/\/scioninstruments.com\/wp-content\/uploads\/2022\/04\/Figure-3-1024x352.jpg 1024w, https:\/\/scioninstruments.com\/wp-content\/uploads\/2022\/04\/Figure-3-300x103.jpg 300w, https:\/\/scioninstruments.com\/wp-content\/uploads\/2022\/04\/Figure-3-768x264.jpg 768w, https:\/\/scioninstruments.com\/wp-content\/uploads\/2022\/04\/Figure-3.jpg 1299w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/a><\/p>\n<p><b>Figure 4. Linearity and accuracy is maintained\u00a0 at scan times of 4ms<\/b><\/p>\n<h2>Analysis of 450 pesticides in blueberries<\/h2>\n<p>A method has been established to identify and quantify 450 analytes from blueberries. The method uses more 1000 MRM transitions over 12 minutes, leading to transitions below 2.5ms. The GC uses the backflush functionality,, which allows backwards carrier flow at the beginning of the injection to avoid non-amenable GC solvents entering the column, and preventing low volatility matrix components to reach the source.<\/p>\n<p>The EFC detector was set to \u2018ON\u2019 with a flow rate of 35 ml\/min. The Pressure Pulse was also on (40PSI, 0.65 minute pulse duration). The initial split ratio was held at 30% for 0.5 minutes and then 0% for 3 minutes, before switching to 100%. The starting temperature of the GC oven was 70<sup>o<\/sup>C for 0.5 minutes, ramping to 300<sup>o<\/sup>C at a rate of 200<sup>o<\/sup>C\/minute and held for 14.3 minutes. The Backflush option was activated with a pressure of 5PSI and a start time of 14 minutes.<\/p>\n<p><a href=\"https:\/\/scioninstruments.com\/wp-content\/uploads\/2022\/04\/Figure-4-1.png\"><img loading=\"lazy\" decoding=\"async\" loading=\"lazy\" class=\"size-large wp-image-46602 alignnone\" src=\"https:\/\/scioninstruments.com\/wp-content\/uploads\/2022\/04\/Figure-4-1-1024x950.png\" alt=\"\" width=\"1024\" height=\"950\" srcset=\"https:\/\/scioninstruments.com\/wp-content\/uploads\/2022\/04\/Figure-4-1-1024x950.png 1024w, https:\/\/scioninstruments.com\/wp-content\/uploads\/2022\/04\/Figure-4-1-300x278.png 300w, https:\/\/scioninstruments.com\/wp-content\/uploads\/2022\/04\/Figure-4-1-768x712.png 768w, https:\/\/scioninstruments.com\/wp-content\/uploads\/2022\/04\/Figure-4-1.png 1184w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/a><\/p>\n<p><b>Figure 5. Calibration and QC results<\/b><\/p>\n<h2>Results<\/h2>\n<p>The data showed excellent linearity for standards and QC samples (Figure 5) with perfect ion scores observed throughout the batch. Retention time reproducibility was extremely consistent and good recovery and reproducibility. This reproducibility was consistent through all of the detected analytes. For example,\u00a0 chlorphenvinfos, an organophosphorous compound that is widely used as an insecticide, was detected at a concentration of 8ppb. The retention time of the compound was 8.45 minutes, which is within 0.02 minutes of the expected RT. The calibration curve had a R<sup>2<\/sup> of &gt;0.998, with a residual of less than \u00b1 20%.<\/p>\n<p><a href=\"https:\/\/scioninstruments.com\/wp-content\/uploads\/2022\/04\/Figure-5-1.png\"><img loading=\"lazy\" decoding=\"async\" loading=\"lazy\" class=\"size-large wp-image-46608 alignnone\" src=\"https:\/\/scioninstruments.com\/wp-content\/uploads\/2022\/04\/Figure-5-1-1024x823.png\" alt=\"\" width=\"1024\" height=\"823\" srcset=\"https:\/\/scioninstruments.com\/wp-content\/uploads\/2022\/04\/Figure-5-1-1024x823.png 1024w, https:\/\/scioninstruments.com\/wp-content\/uploads\/2022\/04\/Figure-5-1-300x241.png 300w, https:\/\/scioninstruments.com\/wp-content\/uploads\/2022\/04\/Figure-5-1-768x617.png 768w, https:\/\/scioninstruments.com\/wp-content\/uploads\/2022\/04\/Figure-5-1.png 1249w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/a><\/p>\n<p><b>Figure 6. Reproducibility of pesticide analysis<\/b><\/p>\n<h2>Summary<\/h2>\n<p>The SCION 8900 TQMS opens up a new possibility for rapid multiplexed screening of pesticides without compromise on sensitivity or accuracy.<\/p>\n<h2>Download Application Note<\/h2>\n<p>Download complete Application Note here: <a href=\"https:\/\/scioninstruments.com\/wp-content\/uploads\/2024\/02\/AN-Multiplexed-pesticide-analysis-by-GC-TQMS.pdf\">Multiplexed pesticide analysis by GC-TQMS<\/a><\/p>\n<h2>Keep in Touch<\/h2>\n<p>If you wish to keep up to date with SCION Instruments latest research and articles, why not join us on social media and sign up to our newsletters today?<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Introduction As plant protection products, pesticides may be used in food production (growth), storage, and transport. The potential physico-chemical transfer of regulated pesticides\u00a0 varies with the type of pesticide used and the type of food being produced.\u00a0 The maximum residue levels (MRLs) of pesticides established by the European Commission in effort to protect consumer safety [&hellip;]<\/p>\n","protected":false},"author":15,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-46571","page","type-page","status-publish","hentry"],"acf":{"rf_hero_height":"default","rf_hero_type":"default","rf_hero_vertpos":"50","rf_hero_horizpos":"50","rf_hero_title":"","rf_hero_title_hide":false,"rf_hero_textcol":"light","rf_hero_bgcol":"white","rf_hero_tint":false,"rf_sitemap_hide":false,"rf_further_reading_posts":false},"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v27.0 (Yoast SEO v27.0) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>Multiplexed pesticide analysis by GC-TQMS | SCION Instruments<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/scioninstruments.com\/us\/multiplexed-pesticide-analysis-by-gc-tqms\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Multiplexed pesticide analysis by GC-TQMS\" \/>\n<meta property=\"og:description\" content=\"Introduction As plant protection products, pesticides may be used in food production (growth), storage, and transport. 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