{"id":35376,"date":"2020-12-11T10:45:09","date_gmt":"2020-12-11T10:45:09","guid":{"rendered":"https:\/\/scioninstruments.com\/determination-of-free-and-total-glycerine-and-mono-di-triglyceride-content-in-b-100-biodiesel-methyl-esters-according-to-en-14105\/"},"modified":"2024-02-08T15:01:54","modified_gmt":"2024-02-08T15:01:54","slug":"determination-of-free-and-total-glycerine-and-mono-di-triglyceride-content-in-b-100-biodiesel-methyl-esters-according-to-en-14105","status":"publish","type":"page","link":"https:\/\/scioninstruments.com\/us\/determination-of-free-and-total-glycerine-and-mono-di-triglyceride-content-in-b-100-biodiesel-methyl-esters-according-to-en-14105\/","title":{"rendered":"Determination of Free and Total Glycerine and mono-, di-, triglyceride content in B-100 Biodiesel Methyl Esters according to EN 14105"},"content":{"rendered":"<h2>Introduction<a href=\"https:\/\/scioninstruments.com\/wp-content\/uploads\/2020\/12\/Untitled-design-7-600x600-1.png\"><img loading=\"lazy\" decoding=\"async\" loading=\"lazy\" class=\"alignright wp-image-34131\" src=\"https:\/\/scioninstruments.com\/wp-content\/uploads\/2020\/12\/Untitled-design-7-600x600-1.png\" alt=\"EN 14105\" width=\"250\" height=\"250\" srcset=\"https:\/\/scioninstruments.com\/wp-content\/uploads\/2020\/12\/Untitled-design-7-600x600-1.png 600w, https:\/\/scioninstruments.com\/wp-content\/uploads\/2020\/12\/Untitled-design-7-600x600-1-300x300.png 300w, https:\/\/scioninstruments.com\/wp-content\/uploads\/2020\/12\/Untitled-design-7-600x600-1-150x150.png 150w\" sizes=\"auto, (max-width: 250px) 100vw, 250px\" \/><\/a><\/h2>\n<p>The European Standard method, <strong>EN-14105<\/strong>, is used to determine the free glycerol and residual mono-, di- and triglyceride contents in fatty acid methyl esters (FAME) typically intended for addition to mineral oils. Total glycerol content is calculated from the results obtained. The method is suitable for FAME from rapeseed, sunflower and soybean oils, but is not suitable for FAME produced from or containing coconut and palm kernel oils due of the problem of peak overlapping. This method and ASTM D6584 are two of the most used standardized analytical methods used for the analysis of biodiesel.<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" loading=\"lazy\" class=\"alignnone wp-image-34125\" src=\"https:\/\/scioninstruments.com\/wp-content\/uploads\/2020\/12\/Fig-1.0-74.png\" alt=\"\" width=\"500\" height=\"267\" srcset=\"https:\/\/scioninstruments.com\/wp-content\/uploads\/2020\/12\/Fig-1.0-74.png 747w, https:\/\/scioninstruments.com\/wp-content\/uploads\/2020\/12\/Fig-1.0-74-300x160.png 300w\" sizes=\"auto, (max-width: 500px) 100vw, 500px\" \/><br \/>\n<em><span style=\"color: #1b66b1;\">Fig. 1<\/span> &#8211; Biodiesel Chromatogram<\/em><\/p>\n<h2>Experimental<\/h2>\n<p>The SCION Biodiesel analyser for EN 14105 is based on our 8300 GC platform, a cold-on-column injector, a SCION-Glycerides Inert Steel analytical column with a 2m retention gap and a High-Temp FID Detector. Analytical conditions for the EN 14105 analysis can be found in table 1.<\/p>\n<table>\n<tbody>\n<tr>\n<td width=\"142\"><strong>Injector <\/strong><\/td>\n<td width=\"189\"><strong>Cold-on-Column <\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"142\">Column<\/td>\n<td width=\"189\">10m x 0.32mm x 0.10\u03bcm<\/p>\n<p>SCION-Glycerides Inert Steel<\/p>\n<p>(SC38613)<\/p>\n<p>w\/ Ret. Gap<\/td>\n<\/tr>\n<tr>\n<td width=\"142\">Oven Program<\/td>\n<td width=\"189\">Start @ 50\u00b0C (1 min)<\/p>\n<p>15\u00b0C\/min to 180\u00b0C<\/p>\n<p>7\u00b0C\/min to 230\u00b0C<\/p>\n<p>10\u00b0C\/min to 370\u00b0C<\/p>\n<p>End @ 370\u00b0C hold 10 min.<\/td>\n<\/tr>\n<tr>\n<td width=\"142\">Carrier<\/td>\n<td width=\"189\">Helium @ 80 kPa<\/td>\n<\/tr>\n<tr>\n<td width=\"142\">Detector<\/td>\n<td width=\"189\">FID @ 380\u00b0C<\/td>\n<\/tr>\n<tr>\n<td width=\"142\">Inj. Volume<\/td>\n<td width=\"189\">1\u00b5l<\/td>\n<\/tr>\n<tr>\n<td width=\"142\">Software<\/td>\n<td width=\"189\">Compass CDS<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><em><span style=\"color: #1b66b1;\">Table 1.<\/span> Analytical conditions<\/em><\/p>\n<h2>Sample Preparation<\/h2>\n<p>Calibration mixtures and internal standard solutions were prepared according to the method.100 mg of homogenized sample was accurately weighed in a 10 mL vial. Then, using a glass syringe, the following was added to the vial: 10\u03bcL of internal standard No. 1 stock solution, 100 \u03bcL of internal standard No. 2 stock solution and 100\u03bcL of MSTFA. Care was taken to ensure there was no contact with moisture. The vials were hermetically sealed and shaken vigorously. After storing the vials at room temperature for ~ 15 minutes, 8 mL of heptane was added to each. Then, 1 \u03bcL of the reaction mixture was automatically injected into the gas chromatograph according to the instrumental conditions described below (see Table 2 for typical results).<\/p>\n<table>\n<tbody>\n<tr>\n<td width=\"76\"><strong>Index<\/strong><\/td>\n<td width=\"132\"><strong>Name<\/strong><\/td>\n<td width=\"113\"><strong>Area (<\/strong><strong>\u00b5V.min)<\/strong><\/td>\n<td width=\"132\"><strong>Quantity (%m\/m)<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"76\"><strong>1<\/strong><\/td>\n<td width=\"132\">Monoglycerides<\/td>\n<td width=\"113\">5692,9<\/td>\n<td width=\"132\">0,69191<\/td>\n<\/tr>\n<tr>\n<td width=\"76\"><strong>2<\/strong><\/td>\n<td width=\"132\">Diglycerides<\/td>\n<td width=\"113\">758,0<\/td>\n<td width=\"132\">0,06116<\/td>\n<\/tr>\n<tr>\n<td width=\"76\"><strong>3<\/strong><\/td>\n<td width=\"132\">Triglycerides<\/td>\n<td width=\"113\">714,6<\/td>\n<td width=\"132\">0,06234<\/td>\n<\/tr>\n<tr>\n<td width=\"76\"><strong>4<\/strong><\/td>\n<td width=\"132\">Glycerin<\/td>\n<td width=\"113\">199,8<\/td>\n<td width=\"132\">0,00975<\/td>\n<\/tr>\n<tr>\n<td width=\"76\"><strong>Total<\/strong><\/td>\n<td width=\"132\"><\/td>\n<td width=\"113\">7365,3<\/td>\n<td width=\"132\">0,82515<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><em><span style=\"color: #1b66b1;\">Table 2.<\/span> Typical results for B-100 Biodiesel<\/em><\/p>\n<h2>Results<\/h2>\n<p>Calibration curves were obtained for glycerin, monoolein, diolein and triolein. Calibration curves for glycerin and monoolein, shown in Figures 2 and 3, are indicative of system performance for the application.<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" loading=\"lazy\" class=\"alignnone wp-image-34173\" src=\"https:\/\/scioninstruments.com\/wp-content\/uploads\/2020\/12\/Fig-2-1.jpg\" alt=\"\" width=\"501\" height=\"252\" srcset=\"https:\/\/scioninstruments.com\/wp-content\/uploads\/2020\/12\/Fig-2-1.jpg 632w, https:\/\/scioninstruments.com\/wp-content\/uploads\/2020\/12\/Fig-2-1-300x151.jpg 300w\" sizes=\"auto, (max-width: 501px) 100vw, 501px\" \/><br \/>\n<em><span style=\"color: #1b66b1;\">Fig. 2<\/span> Biodiesel calibration 001 Glycerin<\/em><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" loading=\"lazy\" class=\"alignnone wp-image-34179\" src=\"https:\/\/scioninstruments.com\/wp-content\/uploads\/2020\/12\/Fig-3.jpg\" alt=\"\" width=\"501\" height=\"248\" srcset=\"https:\/\/scioninstruments.com\/wp-content\/uploads\/2020\/12\/Fig-3.jpg 632w, https:\/\/scioninstruments.com\/wp-content\/uploads\/2020\/12\/Fig-3-300x149.jpg 300w\" sizes=\"auto, (max-width: 501px) 100vw, 501px\" \/><br \/>\n<em><span style=\"color: #1b66b1;\">Fig. 3<\/span> Biodiesel calibration 001 Monoolein<\/em><\/p>\n<table>\n<tbody>\n<tr>\n<td width=\"80\"><strong>File<\/strong><\/td>\n<td width=\"93\"><strong>Glycerin (% m\/m)<\/strong><\/td>\n<td width=\"122\"><strong>Monoglycerides (% m\/m)<\/strong><\/td>\n<td width=\"101\"><strong>Diglycerides (% m\/m)<\/strong><\/td>\n<td width=\"118\"><strong>Triglycerides (% m\/m)<\/strong><\/td>\n<td width=\"104\"><strong>Totals<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"80\"><strong>1<\/strong><\/td>\n<td width=\"93\">0,00975<\/td>\n<td width=\"122\">0,69191<\/td>\n<td width=\"101\">0,06116<\/td>\n<td width=\"118\">0,06234<\/td>\n<td width=\"104\">0,82515<\/td>\n<\/tr>\n<tr>\n<td width=\"80\"><strong>2<\/strong><\/td>\n<td width=\"93\">0,00976<\/td>\n<td width=\"122\">0,71111<\/td>\n<td width=\"101\">0,06304<\/td>\n<td width=\"118\">0,06063<\/td>\n<td width=\"104\">0,84455<\/td>\n<\/tr>\n<tr>\n<td width=\"80\"><strong>3<\/strong><\/td>\n<td width=\"93\">0,00988<\/td>\n<td width=\"122\">0,68955<\/td>\n<td width=\"101\">0,06491<\/td>\n<td width=\"118\">0,06175<\/td>\n<td width=\"104\">0,82618<\/td>\n<\/tr>\n<tr>\n<td width=\"80\"><strong>4<\/strong><\/td>\n<td width=\"93\">0,00977<\/td>\n<td width=\"122\">0,67079<\/td>\n<td width=\"101\">0,06429<\/td>\n<td width=\"118\">0,06165<\/td>\n<td width=\"104\">0,80650<\/td>\n<\/tr>\n<tr>\n<td width=\"80\"><strong>5<\/strong><\/td>\n<td width=\"93\">0,00984<\/td>\n<td width=\"122\">0,67629<\/td>\n<td width=\"101\">0,06290<\/td>\n<td width=\"118\">0,05548<\/td>\n<td width=\"104\">0,80451<\/td>\n<\/tr>\n<tr>\n<td width=\"80\"><strong>6<\/strong><\/td>\n<td width=\"93\">0,00985<\/td>\n<td width=\"122\">0,68240<\/td>\n<td width=\"101\">0,06629<\/td>\n<td width=\"118\">0,05770<\/td>\n<td width=\"104\">0,81624<\/td>\n<\/tr>\n<tr>\n<td width=\"80\"><strong>7<\/strong><\/td>\n<td width=\"93\">0,00980<\/td>\n<td width=\"122\">0,69243<\/td>\n<td width=\"101\">0,06236<\/td>\n<td width=\"118\">0,06057<\/td>\n<td width=\"104\">0,82514<\/td>\n<\/tr>\n<tr>\n<td width=\"80\"><strong>8<\/strong><\/td>\n<td width=\"93\">0,01012<\/td>\n<td width=\"122\">0,60468<\/td>\n<td width=\"101\">0,06489<\/td>\n<td width=\"118\">0,06096<\/td>\n<td width=\"104\">0,74065<\/td>\n<\/tr>\n<tr>\n<td width=\"80\"><strong>9<\/strong><\/td>\n<td width=\"93\">0,00982<\/td>\n<td width=\"122\">0,73039<\/td>\n<td width=\"101\">0,06448<\/td>\n<td width=\"118\">0,06212<\/td>\n<td width=\"104\">0,86680<\/td>\n<\/tr>\n<tr>\n<td width=\"80\"><strong>10<\/strong><\/td>\n<td width=\"93\">0,01014<\/td>\n<td width=\"122\">0,72353<\/td>\n<td width=\"101\">0,06282<\/td>\n<td width=\"118\">0,05948<\/td>\n<td width=\"104\">0,85597<\/td>\n<\/tr>\n<tr>\n<td width=\"80\"><strong>Average<\/strong><\/td>\n<td width=\"93\">0,009883<\/td>\n<td width=\"122\">0,687308<\/td>\n<td width=\"101\">0,0663714<\/td>\n<td width=\"118\">0,060268<\/td>\n<td width=\"104\">0,82117<\/td>\n<\/tr>\n<tr>\n<td width=\"80\"><strong>STDEV<\/strong><\/td>\n<td width=\"93\">0,00015<\/td>\n<td width=\"122\">0,03501<\/td>\n<td width=\"101\">0,00151<\/td>\n<td width=\"118\">0,00218<\/td>\n<td width=\"104\">0,03480<\/td>\n<\/tr>\n<tr>\n<td width=\"80\"><strong>STDEV (%)<\/strong><\/td>\n<td width=\"93\">1,5<\/td>\n<td width=\"122\">5,1<\/td>\n<td width=\"101\">2,4<\/td>\n<td width=\"118\">3,6<\/td>\n<td width=\"104\">4,2<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><em><span style=\"color: #1b66b1;\">Table 3.<\/span> Repeatability results for B-100 Biodiesel<\/em><\/p>\n<h2>Conclusion<\/h2>\n<p>This application note demonstrates the suitability of SCION Instruments Analyser for EN 14105, for the analysis of biodiesel. The calibration curves and repeatability data demonstrate good system integrity. Therefore, the system is well suited to the analysis of free and total glycerol and mono, di and triglyceride content in biodiesel in accordance with the standard method EN 14105.<\/p>\n<h2>Download the Application Note<\/h2>\n<p>You can download the complete application note as a pdf: <a href=\"https:\/\/scioninstruments.com\/wp-content\/uploads\/2024\/02\/AN078-Determination-of-Free-and-Total-Glycerine-and-mono-di-triglyceride-content-in-B-100-Biodiesel-Methyl-Esters-according-to-EN14105.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">Determination of Free and Total Glycerine, mono-, di- and triglyceride content in B-100 Biodiesel Methyl Esters according to EN14105<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Introduction The European Standard method, EN-14105, is used to determine the free glycerol and residual mono-, di- and triglyceride contents in fatty acid methyl esters (FAME) typically intended for addition to mineral oils. Total glycerol content is calculated from the results obtained. The method is suitable for FAME from rapeseed, sunflower and soybean oils, but [&hellip;]<\/p>\n","protected":false},"author":15,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-35376","page","type-page","status-publish","hentry"],"acf":{"rf_hero_height":"default","rf_hero_type":"default","rf_hero_vertpos":"50","rf_hero_horizpos":"50","rf_hero_title":"","rf_hero_textcol":"light","rf_hero_bgcol":"white","rf_hero_tint":false,"rf_hero_title_hide":false,"rf_further_reading_posts":false},"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v27.0 (Yoast SEO v27.0) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>EN14105 - Determination of Glycerine and mono-di-triglyceride in Biodiesel<\/title>\n<meta name=\"description\" content=\"The determination of Free and Total Glycerine, mono-, di- and triglyceride content in B-100 Biodiesel Methyl Esters according to EN14105.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/scioninstruments.com\/us\/determination-of-free-and-total-glycerine-and-mono-di-triglyceride-content-in-b-100-biodiesel-methyl-esters-according-to-en-14105\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Determination of Free and Total Glycerine and mono-, di-, triglyceride content in B-100 Biodiesel Methyl Esters according to EN 14105\" \/>\n<meta property=\"og:description\" content=\"The determination of Free and Total Glycerine, mono-, di- and triglyceride content in B-100 Biodiesel Methyl Esters according to EN14105.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/scioninstruments.com\/determination-of-free-and-total-glycerine-and-mono-di-triglyceride-content-in-b-100-biodiesel-methyl-esters-according-to-en-14105\/\" \/>\n<meta property=\"og:site_name\" content=\"SCION Instruments\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/scioninst\/\" \/>\n<meta property=\"article:modified_time\" content=\"2024-02-08T15:01:54+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/scioninstruments.com\/wp-content\/uploads\/2020\/12\/Untitled-design-7-600x600-1.png\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"4 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/scioninstruments.com\/us\/determination-of-free-and-total-glycerine-and-mono-di-triglyceride-content-in-b-100-biodiesel-methyl-esters-according-to-en-14105\/\",\"url\":\"https:\/\/scioninstruments.com\/determination-of-free-and-total-glycerine-and-mono-di-triglyceride-content-in-b-100-biodiesel-methyl-esters-according-to-en-14105\/\",\"name\":\"EN14105 - 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