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HPLC Analysis of Water-Soluble Vitamins [AN0060]

Vitamins are critical compounds which are essential for normal metabolism function. They are naturally found in many foods but are also often added to processed food products. Additionally, vitamin supplements are a growing trend among people whose diet is restricted. Vitamins are separated into two groups; water soluble and fat soluble. The most common water-soluble vitamin supplements are Thiamine (B1), Pyridoxine (B6), Cyanocobalamin (B12), Riboflavin (B2), Niacin (B3) Nicotinamide, Ascorbic Glucoside, Vitamin C and Erythorbic Acid.
Routine analysis of water-soluble vitamins can be challenging due to the unstable nature of the target analytes. Many factors can affect vitamin stability such as exposure to heat, light, air as well as interactions with other food components. By using reverse phase high pressure liquid chromatography (HPLC) with Diode Array Detector (DAD) a qualitative method for the detection of water-soluble vitamins was easily developed. For quantitative analysis, separate HPLC methods are recommended due to Vitamin C and Erythorbic Acid instability in which decomposition regularly occurs during sample preparation.

LC6000 Analysis of Glycosides in Medicines [AN0067]

Glycosides are molecules in which a sugar is bound to another functional group via a glycosidic bond. Glycosides are mainly O-glycosides, sugar derivatives with various physiological activities that are widely distributed in plants. Additionally, glycosides are widely used as components of unrefined/ herbal medications, such as ginseng or Senna.
SCION Instruments developed a qualitative and quantitative method for the analysis of glycosides in medicines by HPLC-Diode Array Detection (DAD). Confirmation is further certified through the comparison of the absorbance spectrum of both analytical standard and sample analysed.

LC6000_Simultaneous Analysis of Parabens and Phenoxyethanol_2020.1 [AN068]

INTRODUCTION: Parabens are a class of widely used preservatives in cosmetic and pharmaceutical products, primarily due to their bactericidal and fungicidal properties. Parabens are a series of parahydroxybenzoates which are currently under scrutiny due to the possibility that long-term use of products containing parabens can cause hormone disruption and fertility issues, although this has not been confirmed. Methyl phydroxybenzoate, ethyl p-hydroxybenzoate, propyl p-hydroxybenzoate and butyl p-hydroxybenzoate are the most common used parabens in the cosmetic and pharmaceutical industry. Phenoxyethanol is an additional component used as a germicide and antiseptic ingredient in cosmetics. Naturally occurring in plant material such as green tea, phenoxyethanol is often used in tandem with parabens to reduce the number of parabens used during the manufacturing of such products.Monitoring paraben and phenoxyethanol concentrations are a vital part of any cosmetic of pharmaceutical manufacturer.

Optimal Quantification of THC in Blood, May 2019.1 [AN014]

INTRODUCTION: The number of regular cannabis users in the world is estimated at 183 million people[1]. It is illegal to drive a vehicle whilst under the influence of cannabis. The active ingredient in cannabis is delta9-Tetrahydrocannabinol (Δ9-THC). Common side effects includes the feeling of euphoria, hallucinations, relaxation and even sedation. Post ingestion, Δ9-THC is metabolised into the inactive ingredient Tetrahydrocannabinolic Acid (THCA/ THCCOOH).

The Analysis of Residual Solvents by Headspace Sampling and GC According to USP 467: Procedure A, May 2019.1 [AN022]

INTRODUCTION: In the pharmaceutical industry, residual solvents are defined as organic volatile chemicals that are produced during the manufacturing of active pharmaceutical ingredients or derived directly from the packaging of the pharmaceuticals. Residual solvents do not provide any therapeutic benefits and should be removed where possible. Quality assurance laboratories routinely test products for the presence of residual solvents. The United States Pharmacopeia (USP) method 467 is the harmonised test method for the identification and quantification of the organic volatile impurities by gas chromatography. USP 467 details three classes of solvents, in accordance to their health hazards. Additionally, the analytical method is split into two procedures; procedure A is for the identification of the residual solvents at the limit of detection whereas procedure B is for the confirmation of the analyte identity.