Vitamins are critical compounds which are essential for normal metabolism function. They are naturally found in many foods but are also often added to processed food products. Additionally, vitamin supplements are a growing trend among people whose diet is restricted. Vitamins are separated into two groups; water soluble and fat soluble. The most common water-soluble vitamin supplements are Thiamine (B1), Pyridoxine (B6), Cyanocobalamin (B12), Riboflavin (B2), Niacin (B3) Nicotinamide, Ascorbic Glucoside, Vitamin C and Erythorbic Acid.
Routine analysis of water-soluble vitamins can be challenging due to the unstable nature of the target analytes. Many factors can affect vitamin stability such as exposure to heat, light, air as well as interactions with other food components. By using reverse phase high pressure liquid chromatography (HPLC) with Diode Array Detector (DAD) a qualitative method for the detection of water-soluble vitamins was easily developed. For quantitative analysis, separate HPLC methods are recommended due to Vitamin C and Erythorbic Acid instability in which decomposition regularly occurs during sample preparation.